HPLC

High-Pressure Pump

• Flow Rate: Typically ranges from 0.1 mL/min to 10 mL/min, with some high-performance models reaching even higher flow rates.

• Pressure Limit: It depends on the model but commonly ranges from 40 MPa (6,000 psi) to 100 MPa (14,500 psi) to handle high-pressure mobile phases.

Injector

• Injection Volume: Usually between 1 µL and 100 µL, depending on the injector type and sample concentration.

• Reproducibility: Ideally, +/- 1% or better for accurate and consistent sample introduction.

Column

• Length:  Common ranges are 50 mm to 250 mm for analytical columns and longer for preparative HPLC.

• Diameter: Typically between 2.1 mm and 4.6 mm for analytical columns. 

Detector

• Sensitivity: It varies with the type of detector. For instance, the sensitivity of UV/Vis detectors varies according to the wavelength used. 

Data Acquisition System

• Sampling Rate: The latest models have higher sampling rates that capture fast-eluting peaks with better detail. (e.g., 10 Hz to 100 Hz)

• These are general ranges, and specific features will vary depending on the model and intended use of the HPLC system.